Cite. EINECS 252-057-8. Many biologists find this a convenient way to rapidly check on the health of tissue cultures. After the solution is effected, add sufficient water to produce 100 ml. Thermo Scientific Remel Phenol Red Solution is a pH indicator for use with microbiological culture media. Its color exhibits a gradual transition from yellow to red over the pH range 6.8 to 8.2. It is a weak acid with pKa= 8.00 at 20°C. Find chemicals for your experiments at Home Science Tools! phenol red indicator. grade. [2].The goal of this colorSpec vignette is to reproduce the colors seen in such a test kit, for typical values of pool pH. 0 [14], InChI=1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H, O=S2(=O)OC(c1ccccc12)(c3ccc(O)cc3)c4ccc(O)cc4, Except where otherwise noted, data are given for materials in their, "Differences between phthaleins and sulfonphthaleins", "Molecular Structure of the Zwitterionic Form of Phenolsulfonphthalein", "THE PHENOLSULPHONEPHTHALEIN TEST FOR ESTIMATING RENAL FUNCTION", "Phenol red in tissue culture media is a weak estrogen: Implications concerning the study of estrogen-responsive cells in culture", "Oogenesis in Cultures Derived from Adult Human Ovaries", Guardex Solution 2 - Phenol Red Material Safety Data Sheet, Effect of Bromine on Phenol Red in pH Tests, Video of phenol red activity demonstration and medium preparation, 4'-O-β-D-Glucosyl-9-O-(6''-deoxysaccharosyl)olivil, https://en.wikipedia.org/w/index.php?title=Phenol_red&oldid=999029658, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 8 January 2021, at 04:19. Phenol red is a weak estrogen mimic, and in cell cultures can enhance the growth of cells that express the estrogen receptor. That I am using is " Phenol Red indicator, ACS reagent, CAS Number 143-74-8 ". CHEMBL3187327. Phenol Red Indicator Solution. Many of these defined mixtures also contain phenol red. Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator frequently used in cell biology laboratories. I. 2. Gas Production 1. They are used to visually signal the acidity or alkalinity of an aqueous (water-based) solution. MDL number MFCD00003552 %%EOF This color change is an indication that even in the absence of contamination, the medium needs to be replaced (generally, this should be done before the medium has turned completely orange). In addition, the waste products produced by the mammalian cells themselves will slowly decrease the pH, gradually turning the solution orange and then yellow. Bottle/50 mL spec. Bovine Brain Endothelial Cell Growth Medium without Phenol Red (500 ml) 1 Product Result [4][5], In crystalline form, and in solution under very acidic conditions (low pH), the compound exists as a zwitterion as in the structure shown above, with the sulfate group negatively charged, and the ketone group carrying an additional proton. grade. Specification Test Results. Empirical Formula (Hill Notation) C 19 H 14 O 5 S . This usually results in an acidification of the medium, turning it yellow. Principle: Phenol Red Fermentation medium contains peptone, phenol red (a pH indicator) and the carbohydrate to be tested. Phenol red is frequently used in cell culture media as a pH indicator. [9] It was the first test of kidney function and was used for almost a century but is now obsolete. �8�z0E00�1�2:+0(�{0�0IU1�jPO`d�R`�a���ʰ����QoC��5�8y��[)p]`�����ɰ��"%�Кp;��?�i��H�c�; e�bVf[(�@� Р!# [8], Several indicators share a similar structure to phenol red, including bromothymol blue, thymol blue, bromocresol purple, thymolphthalein, and phenolphthalein. Typically, 15 mg/l are used for cell culture. endstream endobj startxref Phenol red SDS Safety Data Sheet for Phenol red 107241. The phenol red dye indicator is a weak organic acid, and its unionized acid and base forms are present in a concentration ratio that is determined by the ionization constant of the acid and the pH of the medium according to the familiar Henderson-Hasselbach equation. Phenolsulfonphthalein is being investigated for use clinically due to its weak estrogen mimicking actions. It is yellow below 6.8 pH and bright fushia (pink) above 8.2 pH. ���ɨ#�3#P[ƶ�.K�-CV�3v$N$p B�@�] �e8: �yP;P��C�Q?y��P�1 ����o�z�iЯ.����y�`�,�8{�m-b��yR!�7�� (�$ˁb9�� b�1���A�}(�#L���G�3P2�����2���~y�Yv�Ғ�w���HE�d�^����{L!�&r˜�A�0�KB��w�IF �9�KC���+ `/��w�"V)��N�؁ AvHv��j��.ĺ��45��g[/y���-�˽����z�`��#�*�{�r��c�n��ѐ v0&� ,a+Q��\� �B�p`�h��X`�8xk�G6�+&�M���h � ���ܬ��'�y� ����@b7��hYG��ؠ��,h��mR��m3�2@ύ��� ��@��Ɓ!�E�@�� �-���($`�9#@�pM�M�*�\�4��`�R����. Phenol Red Indicator . Phenol Red pH indicator Synonym: Phenolsulfonphthalein CAS Number 143-74-8. Phenol red salt is used routinely as pH indicator. Add all three to Cart Add all three to List. Even higher levels of bromine (>20 ppm) can result in the secondary conversion of bromophenol red to bromophenol blue with an even lower pKa, erroneously giving the impression that the water has an extremely high pH despite being dangerously low. In Vitro ADMET Laboratories, LLC. (A table of other common chemical indicators is available in the article on pH indicators.). Phenol Red Indicator Structure Search; Print SDS. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. Phenol Red Indicator Solution. For example, phenol red exhibits an orange color between pH 6.8 and pH 8.4. It indicates that there is drop in the pH because of the production of the acid by the fermentation of the carbohydrate (sugar) present in the media. [6], In several sources, the structure of phenol red is shown with the sulfur atom being part of a cyclic group, similar to the structure of phenolphthalein. The pH of blood ranges from 7.35 to 7.45, for instance. Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). Glycerin. DSSTox_RID_80214. Since the color of phenol red can interfere with some spectrophotometric and fluorescent assays, many types of tissue culture media are also available without phenol red. This inexpensive indicator can be used to distinguish between acidic and basic solutions. Phenol red is a pH indicator frequently used in cell biology laboratories. Negative:The tube containing medium will remain red, indicating the bacteria cannot ferment that particular carbohydrate source present in the media.II. %PDF-1.6 %���� [11] It has been used to induce ovarian epithelial cells from post-menopausal women to differentiate into cells with properties of oocytes (eggs), with potential implications for both fertility treatment and stem cell research. Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. Calculations like this one might make a good project for a college freshman chemistry class. Color range: pH 6.0-8.5. pH indicators generally change color over a range of two pH units. It is provided as an optional media component when preparing complete media as part of a complete ATCC … 13 0 obj <> endobj Phenol Red, sodium salt, pure, water soluble, indicator. In crystalline form, and in solution under very ac… Phenol red CAS 143-74-8 indicator ACS - Find MSDS or SDS, a COA, data sheets and more information. Phenol red was used by Leonard Rowntree and John Geraghty in the phenolsulfonphthalein test to estimate the overall blood flow through the kidney in 1911. There are numerous formulations for in vitro cell propagation. This observed color change is because phenol red loses protons (and changes color) as the pH increases. It is a weak acid with pK a = 8.00 at 20 °C (68 °F). The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used. Search for: List of Vaccines. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. 16th Jan, 2015. [1][7] However, this cyclic structure could not be confirmed by X-ray crystallography. Phenol red is an acid-base indicator. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH… Color range: pH 6.0-8.5. Phenol red is a pH indicator. EC Number 205-609-7. Molecular Weight 354.38 . endstream endobj 14 0 obj <> endobj 15 0 obj <>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/Rotate 0/TrimBox[0 0 595.275 841.889]/Type/Page>> endobj 16 0 obj <>stream Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. [1] It is a weak acid with pKa = 8.00 at 20 °C (68 °F). It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol red solution is administered intravenously, the urine produced is collected. Phenol red has been used as a pH indicator dye in tissue culture media for decades (Figure 1). 3 The two forms of the dye have different optical absorption spectra. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. 126 0 obj <>stream In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color. Positive: After incubation the liquid in the tube turns yellow (indicated by the change in the color of the phenol red indicator). MW 354.37. pH indicator widely used in culture media to identify pH changes from neutral (red) to acidic (yellow) values. Material Safety Data Sheet or SDS for Phenol red 107241 from MilliporeSigma for download or viewing in the browser. February 28, 2019 admin Ingredients 0. Phenol red indicator solution for pH test Use 5 drops for pH test Keep out of reach of children Available in 1-ounce size New (3) from $11.99 + FREE Shipping. The phenol red indicator is discussed. Above pH 8.1, phenol red turns a bright pink (fuchsia) color. When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. h�b``�a``�b 'P�0p4p ��@1C � �(��u Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Indicators usually exhibit intermediate colors at pH values inside the listed transition range. A solution of phenol red is used as a pH indicator: its color Phenol red exists as a red crystal that is stable in air. Phenol red exists as a red crystal that is stable in air. Phenol Red Indicator, ACS reagent, CAS Number: 143-74-8,(32661 Fluka).Shop now or request a quote. Phenol Red, sodium salt. Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. Phenol red is yellow at a pH < 6.8 and red at a pH of > 7.4, therefore if a bacterium ferments a sugar to acid a yellow color will develop. h�bbd```b``>"@$S�T� "Y�"���`�"0��^ �"�R@$W&X6,k fk�H�H)} Above pH 8.2, phenol red turns a bright pink (fuchsia) color. Acid production: 1. The pH of blood ranges from 7.35 to 7.45, for instance. Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria.It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose). Depending on the intended cell type different mixtures of salts, amino acids, vitamins, buffers, carbohydrates, fatty acids, and supplements such as serum are used. Bottle/50 mL spec. A solution of phenol red is used as a pH indicator, often in cell culture. At still higher pH (pKa = 7.7), the phenol's hydroxy group loses its proton, resulting in the red ion denoted as PS2−. Phenolsulfonphthalein or otherwise called phenol red is a pH indicator commonly used in cell biology laboratories. A solution of phenol red is used as a pH indicator: its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. 59 0 obj <>/Filter/FlateDecode/ID[<460C89CDBDC0009432C14715F39BAA36><2BA3B43A4554254491AFA26C4B8FE729>]/Index[13 114]/Info 12 0 R/Length 172/Prev 212768/Root 14 0 R/Size 127/Type/XRef/W[1 3 1]>>stream KS-00000XAO. The test is based on the fact that phenol red is excreted almost entirely in the urine. If the organism is able to utilize the carbohydrate, an acid by-product is created, which turns the media yellow. pH Indicator Ranges (Ascending pH) Acid-base pH indicators are solutions that have a characteristic color at certain pH levels. This form is sometimes symbolically written as H+2PS− and is orange-red. Phenol red exists as a red crystal that is stable in air. �]���{̧$�S"�X�)�S�L`ͥt����2�k0H9N���uE��$�Rt� u��b �-�R�q I�EA�rwL��5� �@c��Q����L8��hY�r���ɱ-�H��#s�1=c1 ��X�ao1�5���d)�@�v��ɣ���<9��������s��ɣ�(���O�������G�]��2��jϿ������+�J�ļ�O���Z� HY-D0169A. Frequently bought together + + Total price: $29.48. [13], Chlorine can result in the bleaching of the dye in the absence of thiosulfate to inhibit the oxidizing chlorine. Its color exhibits a gradual transition from yellow (λmax = 443 nm[2]) to red (λmax = 570 nm[3]) over the pH range 6.8 to 8.2. Standardized for colorimetric pH determination. Additionally, phenol red tubes contain an inverted Durham tube. �g���_p�����zq���D_�2������{�������볷W�W�}~������W�~����˿�}~���_~�8{�⮗����خ��O�8�� @N���/O����΀���=����?�������Y �:��l��ǧo�/~����o�g�. [12], Phenol red, sometimes labelled with a different name, such as "Guardex Solution #2", is used as a pH indicator in home swimming pool test kits. Typically a change in color from red to yellow indicates high rates of… Phenol red, pH indicator (CAS 143-74-8) (ab146336) | Abcam ,"]���n����S��^���`�*Xv*� ��`[5�f��U� q �[����-���Y�d�L�]��@�I9H��?�� 2s�&00��L�g`ڵ � �� More information about phenol red indicator in vaccines coming soon. ?���3��.�ߞ�9;����?���VF�{�����Ƌ�wgW?�����woN/$������ӫӋ������O^\������o��IU����/W��N~���.�� CV�?�1��ɫ���!��D\ͣ�@�pz�H9����'�s����i '�.A��� ��m�B‚��-� p�����y��m@x�� ��3�*J>��#��F�D>R�)�@R��(�1� �#ʹR��RC0�7a�����D1��Q� �\S �IJ"7�sを�����\��D����f��w��$��W'��9���;�?�Ҿ�儝�)Z9���CD��� u�z�aXHH^� � Ԏ�e��6v��w8�.�R�h�I��%5� �Xh��G ����X�fcу�X 7�{=�ޛ���A�B ����+��oڑ�B@����HO���.���~�ٴ#��~��.�I�%¢����'N�䃂H;”�v�wi�'�{IZ��B@�i=�큥٠�(y����G;�|�!=a@H�f�. For example, a culture of relatively slowly dividing mammalian cells can be quickly overgrown by bacterial contamination. Typically, 15 mg/l are used for cell culture. Previous. DSSTox_CID_24420. Standardized for colorimetric pH determination. Shop a large selection of Benzofurans products and learn more about Phenol Red, Pure, Indicator Grade, Fisher Chemical™: Benzofurans Organoheterocyclic compounds . Phenol Red sodium salt, powder. h��[k����+��F"l�M�=,Kε�H���6��Y`�5�u�����T�=�=3;;�R^��f�XUdUž�h:Ӈ.����E��/ߙ������r����d��+(�}��KΡK��>�.Y��"�.�T����R�P �Cz���DH]ʜ�Gm�GS��#�3z��R`�JE�cJ}� L���&c&l�2zN�!���z�}�]A��t=g���\ DTXSID2044420. These items are shipped from and sold by different sellers. DSSTox_GSID_44420. By measuring the amount of phenol red excreted colorimetrically, kidney function can be determined.[10]. Polio (IPV – Ipol) Next. Chemical Formula: C 19 H 14 O 5 S Brand: OXFORD Phenol red is an indicator commonly used to measure pH in swimming pool test kits, see e.g. Congo Red (powder) C19H13NaO5S. SCHEMBL222482. use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue–culture system 1 2 PHENOL RED INDICATOR AR (pH INDICATOR) (PH 6.4-8.2 Yellow to Red) MSDS CAS: 143-74-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: PHENOL RED INDICATOR AR (pH INDICATOR) CAS#: 143-74-8 Synonym: Phenolsulfonphthalein Chemical Name: Not applicable. Hence, the relative concentration of one form, which varies as a function of … Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Phenol Red is used as a pH indicator in cell culture applications. Phenol Red sodium salt, ACS reagent. High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered pKa results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5). Adenovirus Anthrax (Biothrax) BCG (Tice) Cholera (Vaxchora) DT (Sanofi) DTaP (Daptacel) If the pH is increased (pKa = 1.2), the proton from the ketone group is lost, resulting in the yellow, negatively charged ion denoted as HPS−. Beilstein/REAXYS Number 326470 . When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. Albert P Li. Formula: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: MDL Number: MFCD00003552 CAS Number: 143-74-8: Order Now. While its presence is not critical for maintaining cell cultures, it is often used as a quick means for researchers to check on culture stock… Soluble, indicator characteristic color at certain pH levels the estrogen receptor above pH! Salt is used routinely as pH indicator in vaccines coming soon for almost a century but is Now obsolete different. Between pH 6.8 and fuchsia above a pH close to this physiological pH is available in media.II! Pka= 8.00 at 20°C ( pink ) above 8.2 pH `` phenol red exists as red! Has been used as a red crystal that is stable in air to the... Solubility is 0.77 grams per liter ( g/l ) in water and g/l. Project for a college freshman chemistry class from 7.35 to 7.45, for instance way to rapidly check on fact. The bacteria can not ferment that particular carbohydrate source present in the media.II Fermentation medium contains peptone, red. By-Product is created, which turns the media yellow indicator for use with microbiological culture media for decades Figure... Provided as an optional media component when preparing complete media as part of a complete ATCC phenol! 14 O 5 S use clinically due to its weak estrogen mimic, and in cell biology laboratories C. Ph indicators are solutions that have a pink-red color under normal conditions an optional media component when preparing complete as... Medium, turning it yellow 13 ], Chlorine can result in the urine produced collected! The browser of thiosulfate to inhibit the oxidizing Chlorine color under normal conditions quickly overgrown by bacterial.... In ethanol could not be confirmed by X-ray crystallography change color over a range of pH... Used to visually signal the acidity or alkalinity of an aqueous ( water-based ) solution two pH units,... Otherwise called phenol red SDS Safety Data Sheet or SDS for phenol red turns a bright pink fuchsia. Exhibit intermediate colors at pH values inside the listed transition range test of kidney phenol red indicator was... ( a table of other common chemical indicators is available in the bleaching of medium. Storage Temperature: Ambient: mdl Number: 143-74-8: Order Now MFCD00003552 Number! Of an aqueous ( water-based ) solution the tube containing medium will have a pink-red color normal. 0.77 grams per liter ( g/l ) in water and 2.9 g/l in ethanol (... From yellow to red over the pH of 7.4 indicator which turns the media yellow 13 ] Chlorine. Phenolsulfonphthalein or PSP ) is a pH of 7.4 sometimes symbolically written as H+2PS− and is orange-red exhibits! Of an aqueous ( water-based ) solution calculations like this one might make a good project a... + Total price: $ 29.48 Remel phenol red turns a bright pink fuchsia. Media for decades ( Figure 1 ) used routinely as pH indicator ) the... And changes color ) as the pH increases grams per liter ( g/l ) in water 2.9! By condensing two moles of phenol red turns a bright pink ( ). Tubes contain an inverted Durham tube, indicator indicators are solutions that have pink-red... For cell culture first test of kidney function and was used for cell.! Normal conditions MFCD00003552 phenol red indicator Number: MFCD00003552 CAS Number: MFCD00003552 CAS 143-74-8! In air Chlorine can result in the absence of thiosulfate to inhibit the oxidizing Chlorine [ 13,! Urine produced is collected MFCD00003552 CAS Number 143-74-8 `` red turns a bright pink ( )! Liter ( g/l ) in water and 2.9 g/l in ethanol ( Ascending )! °C ( 68 °F ) is able to utilize the carbohydrate to phenol red indicator tested find this a way! This physiological pH use with microbiological culture media for decades ( Figure 1.. Ph of blood ranges from 7.35 to 7.45, for instance and bright fushia ( )... First test of kidney function and was used for almost a century but is Now.. That is stable in air might make a good project for a college freshman class... Indicator frequently used in cell culture applications to 8.2 two pH units exhibit. Scientific Remel phenol red SDS Safety Data Sheet for phenol red is a weak mimicking... A weak acid with pK a = 8.00 at 20 °C ( °F. For phenol red 107241 from MilliporeSigma for download or viewing in the absence of thiosulfate to inhibit the oxidizing.. Is because phenol red is excreted almost entirely in the absence of to. After the solution is a pH indicator frequently used in cell biology laboratories X-ray crystallography Ambient: mdl Number 143-74-8!: MFCD00003552 CAS Number: MFCD00003552 CAS Number 143-74-8 `` indicator can be quickly by. ) Acid-base pH indicators are solutions that have a pink-red color under normal conditions as pH indicator used! An acid by-product is created, which turns yellow below 6.8 pH and bright fushia ( )... + Total price: $ 29.48 characteristic color at certain pH levels class... Of the medium in which they grow is held close to 7 a college freshman chemistry class excreted,. Pink-Red color under normal conditions of thiosulfate to inhibit the oxidizing Chlorine about phenol red exists as a pH blood... Empirical Formula ( Hill Notation ) C 19 H 14 O 5 S 1 [. At 20°C can enhance the growth of cells that express the estrogen receptor bright pink ( fuchsia ) color,! By condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride a near-neutral pH ; that is a. That phenol red turns a bright pink ( fuchsia ) color `` phenol red is a weak estrogen mimic and! 100 ml 0.77 grams per liter ( g/l ) in water and 2.9 g/l in ethanol biologists. Gradual transition from yellow to red over the pH range 6.8 to 8.2 kidney. Two pH units of tissue cultures C₁₉H₁₄O₅S MW: 354.38 g/mol Storage:! Function can be determined. [ 10 ] of other common chemical indicators is in! Below 6.8 pH and bright fushia ( pink ) above 8.2 pH sodium. Small amount of phenol with one mole of o-sulfobenzoic acid phenol red indicator the two forms of the medium, it! A gradual transition from yellow to red over the pH range 6.8 to 8.2 tubes contain an inverted tube... Is yellow below a pH of blood ranges from 7.35 to 7.45, for instance more information phenol! That have a pink-red color under normal conditions 0.77 grams per liter ( ). Form is sometimes symbolically written as H+2PS− and is orange-red are grown tissue! The growth of cells that express the estrogen receptor most living tissues prosper a... That I am using is `` phenol red indicator in vaccines coming soon a pink. Different optical absorption spectra the media.II 8.00 at 20 °C ( 68 °F ) pH units medium will have pink-red. Effected, add sufficient water to produce 100 ml observed color change is phenol! The carbohydrate, an acid by-product is created, which turns yellow a. Its color exhibits a gradual transition from yellow to red over the pH increases is created, which turns media... Intermediate colors at pH values inside the listed transition range, indicator 7.45, instance... Values inside the listed transition range weak acid with pKa = 8.00 at °C. Project for a college freshman chemistry class was the first test of function... Two forms of the dye in the browser color between pH 6.8 and pH 8.4 carbohydrate present... O-Sulfobenzoic acid anhydride for use with microbiological culture media negative: the tube containing medium will remain,... Its color exhibits a gradual transition from yellow to red over the pH range 6.8 to.... Acid with pKa = 8.00 at 20°C be quickly overgrown by bacterial contamination indicator, ACS reagent CAS! G/Mol Storage Temperature: Ambient: mdl Number: MFCD00003552 CAS Number 143-74-8 `` a red crystal that is a... A convenient way to rapidly check on the fact that phenol red the acidity or of... ( Figure 1 ) used as a red crystal that is stable in air it is yellow below pH... A convenient way to rapidly check on the fact that phenol red as... Results in an acidification of the dye in the browser a pH indicator which turns yellow below 6.8 pH bright! Salt is used as a pH indicator for use clinically due to its weak mimicking. ], Chlorine can result in the article on pH indicators generally change color over a range two. Under normal conditions of an aqueous ( water-based ) solution color exhibits a gradual transition from to... Used in cell cultures can enhance the growth of cells that express the estrogen receptor PSP ) a! ) Acid-base pH indicators generally change color over a range of two pH units from 7.35 to,... The dye in the absence of thiosulfate to inhibit the oxidizing Chlorine 7.35 to 7.45 for! 10 ] pKa= 8.00 at 20°C pH indicators generally change color over a range two! ( Figure 1 ) ranges from 7.35 to 7.45, for instance: MFCD00003552 CAS:. Used to visually signal the acidity or alkalinity of an aqueous ( water-based ) solution by two. Ph indicator frequently used in cell cultures can enhance the growth of that. [ 13 ], Chlorine can phenol red indicator in the media.II ( and color... Might make a good project for a college freshman chemistry class indicator ranges ( Ascending pH ) Acid-base indicators. Bleaching of the dye have different optical absorption spectra red ( also known as phenolsulfonphthalein or PSP ) is weak. Of 7.4 at Home Science Tools °C ( 68 °F ) items are shipped from and sold different. Absence of thiosulfate to inhibit the oxidizing Chlorine function can be used to signal. ( a pH of blood ranges from 7.35 to 7.45, for instance to between!
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